Western blot analysis was performed as we previously reported [30 (link)]. Cells were seeded in 6-well plates. After 24 h, the cells in each well were treated with HCQ or Spautin-1 for 1 h and then BKM120 for 48 h. Total proteins were separated and blotted. The signal was detected by a ChemiDocXRS+ System (BIO-RAD) after exposure to chemiluminescence reagents (BIO-RAD). β-actin served as the loading control.
Free full text: Click here