Inflammation is a further consideration to take into account after stent deployment. Nanomatrix coated stainless steel strips demonstrated excellent attenuation of inflammatory responses as previously reported.25 (link) Here, stents were utilized to better recapitulate clinical conditions. Stents were coated with PA-YK-NO or left uncoated as described above. U937 monocytes (1 × 106 cells/mL) labeled with CellTracker Green CMFDA (Life Technologies) were perfused over the stents at 10 dyn/cm2 along with TNF-α (10 ng/mL) for 4 h to stimulate inflammatory conditions. After 4 h, stents were removed from the bioreactor and washed twice with warm sterile PBS to remove loosely bound monocytes. Monocyte adhesion was analyzed using Nikon NIS Elements imaging software (Melville, NY), and the percent of the stent strut face area covered by adherent monocytes was calculated using ImageJ (NIH).
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Stent-Induced Inflammatory Response Attenuation
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Other organizations : University of Alabama at Birmingham, Emory University, Yonsei University
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Variable analysis
independent variables
- Stent coating: PA-YK-NO or uncoated
- Percent of the stent strut face area covered by adherent monocytes
- Stent deployment to recapitulate clinical conditions
- U937 monocytes (1 × 10^6 cells/mL) labeled with CellTracker Green CMFDA (Life Technologies)
- Perfusion of monocytes over the stents at 10 dyn/cm^2 along with TNF-α (10 ng/mL) for 4 h to stimulate inflammatory conditions
- Washing of stents twice with warm sterile PBS to remove loosely bound monocytes
- Positive control: Not explicitly mentioned
- Negative control: Uncoated stents
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