Box-structured meshes (4 × 4 cm2) composed of polycaprolactone (Purasorb PC 12, Corbion Inc., Gorinchem, Netherlands) were fabricated with 70 layers (1 mm height) of overlaying fibers (layered in orthogonal directions) as previously described [40 (link)]. A custom-built MEW device equipped with an electrical heating system (TR 400, HKEtec, Germany; heating temperature = 90 °C) was used to feed PCL polymer melt (feed pressure = 3 bar) through a 23G spinneret charged by a high voltage power supply (LNC 10000–5 pos, Heinzinger Electronic GmbH, Rosenheim, Germany). Processed PCL fibers (diameter ~20 μm) were then collected on a computer-controlled collector plate (acceleration voltage=5.5 kV, spinning gap= 3.3 mm, E = 1.3 kV/mm). Each mesh was fabricated with a 90° lay-down pattern and the spacing between deposited fibers was 200μm, 400 μm or 800 μm. Disc-shaped mesh constructs were obtained from printed 1 mm thick MEW meshes using a 4 mm biopsy punch.
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Fabrication of PCL Meshes for Tissue Engineering
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Corresponding Organization : University of Pennsylvania
Other organizations : Philadelphia VA Medical Center, University Medical Center Utrecht, Eindhoven University of Technology, Utrecht University
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Variable analysis
independent variables
- Spacing between deposited fibers (200 μm, 400 μm or 800 μm)
- Fiber diameter (~20 μm)
- Polycaprolactone (Purasorb PC 12, Corbion Inc., Gorinchem, Netherlands) as the material
- 70 layers (1 mm height) of overlaying fibers fabricated
- Laydown pattern of 90°
- Feed pressure = 3 bar
- Acceleration voltage = 5.5 kV
- Spinning gap = 3.3 mm
- Electric field strength = 1.3 kV/mm
- Heating temperature = 90 °C
- No positive or negative controls were explicitly mentioned.
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