miRNA target prediction tools, including PicTar version 2007 (https://pictar.mdc-berlin.de/ ) and TargetScan Release 7.0 (http://targetscan.org/ ) were used to search for the putative targets of miR-17. The dual luciferase reporter assay was performed as described previously (25 (link)). RAW264.7 cells were treated with miR-17 mimics, miR-17 inhibitor the luciferase reporter plasmids (wt-TLR4-UTR-pGL3 or mt-TLR4-UTR-pGL3), and pRL-TK-Renilla control plasmid (Promega Corporation) using Lipofectamine 2000 (Invitrogen; Thermo Fisher Scientific, Inc.). At 48 h post-transfection, lucif-erase activity was detected using the Dual Luciferase Reporter kit (Beyotime Institute of Biotechnology).
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MiR-17 Targets TLR4 Regulation
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Corresponding Organization : Inner Mongolia University for Nationalities
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Variable analysis
independent variables
- MiR-17 mimics
- MiR-17 inhibitor
- Luciferase activity
- Wt-TLR4-UTR-pGL3 or mt-TLR4-UTR-pGL3 luciferase reporter plasmids
- PRL-TK-Renilla control plasmid
- Not explicitly mentioned
- Not explicitly mentioned
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