M. tuberculosis isolates were obtained from a previous collection of samples from 1 Swedish patient during 9 years (1991–1999) of pulmonary tuberculosis [20 (link)]. The high sequence similarity strongly suggests that the patient carried the same single clone during 9 years without reinfection. The samples (populations) were collected at 8 different time points before and during treatment. Each sample was grown on Löwenstein-Jensen slants at 37°C to subsequently isolate up to 10 single colonies. The isolates were analyzed for phenotypic drug susceptibility using the BACTEC 460TB system (Becton and Dickinson). Both populations and individual isolates were kept frozen at −70°C until used in this study.
Protocol detail
Long-term Tracking of M. tuberculosis Clone
M. tuberculosis isolates were obtained from a previous collection of samples from 1 Swedish patient during 9 years (1991–1999) of pulmonary tuberculosis [20 (link)]. The high sequence similarity strongly suggests that the patient carried the same single clone during 9 years without reinfection. The samples (populations) were collected at 8 different time points before and during treatment. Each sample was grown on Löwenstein-Jensen slants at 37°C to subsequently isolate up to 10 single colonies. The isolates were analyzed for phenotypic drug susceptibility using the BACTEC 460TB system (Becton and Dickinson). Both populations and individual isolates were kept frozen at −70°C until used in this study.
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Clone
Drug
Frozen
M tuberculosis
Patient
Phenotypic
Populations
Pulmonary tuberculosis
Reinfection
Samples collection
Susceptibility
Corresponding Organization : Uppsala University
Other organizations : Public Health Agency of Sweden, Karolinska Institutet
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Variable analysis
independent variables
- Time points of sample collection (before and during treatment)
- Phenotypic drug susceptibility of M. tuberculosis isolates
- Growth conditions (Löwenstein-Jensen slants at 37°C)
- Sample storage (-70°C)
- Positive control: None mentioned
- Negative control: None mentioned
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