Human hepatoma cell lines (HepG2 and Huh7), hepatic stellate cell line LX-2 and human pancreatic cancer MIAPaCa-2 cells were maintained in Roswell Park Memorial Institute medium (RPMI 1640) (Sigma, St. Louis, MO, USA) supplemented with 1-10% fetal bovine serum, penicillin (100 U/mL) and streptomycin (100 μg/mL) at 5% CO2 and 37°C. HepG2, Huh7 and MIAPaCa-2 cells were purchased from the Japanese Collection of Research Bioresources Cell Bank (Ibaraki, Osaka, Japan) 26 (link), 51 (link). LX-2 cells, spontaneously immortalized cells, were kindly provided by Prof. Scott L. Friedman, Mount Sinai Medical School, NY, USA 52 (link). Oleic acid-albumin from bovine serum was purchased from Sigma.
Protocol detail
Culturing Hepatic and Pancreatic Cell Lines
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Bovine serum albumin
Cell lines
Cells
Fetal bovine serum
Hepatic stellate cell
Hepatoma
Human
Japanese
Oleic acid
Pancreatic cancer
Penicillin
Streptomycin
Corresponding Organization :
Other organizations : Nihon University
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Variable analysis
independent variables
- Oleic acid-albumin from bovine serum
- Outcomes of experiments conducted on human hepatoma cell lines (HepG2 and Huh7), hepatic stellate cell line LX-2, and human pancreatic cancer MIAPaCa-2 cells
- Roswell Park Memorial Institute medium (RPMI 1640) (Sigma, St. Louis, MO, USA) supplemented with 1-10% fetal bovine serum, penicillin (100 U/mL) and streptomycin (100 μg/mL)
- 5% CO2 and 37°C incubation conditions
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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