On P7 and P14, rat pups were anesthetized, and their lungs were harvested and prepared for immunohistochemistry and histological analyses, as previously described (14 (link),33 (link)). For immunohistochemical evaluation of von Willebrand factor (vWF), the deparaffinized lung sections were incubated with polyclonal rabbit antihuman vWF antibody (DakoCytomation, Glostrup, Denmark) and a biotin-labeled donkey antimouse secondary antibody (Jackson ImmunoResearch Laboratories, West Grove, PA). Immune complexes were visualized using diaminobenzidine (Vector Laboratories, Burlingame, CA). Light hematoxylin and eosin was applied as a counterstain.
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Immunohistochemical Analysis of Lung Vasculature
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Antibody
Biotin
Donkey
Eosin
Hematoxylin
Immune complexes
Immunohistochemistry
Light
Lung
Rabbit
Vector
Von willebrand factor
Corresponding Organization :
Other organizations : Seoul National University, Seoul National University Bundang Hospital, Wonkwang University, Hanyang University
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Variable analysis
independent variables
- Day of rat pup development (P7, P14)
- Immunohistochemical evaluation of von Willebrand factor (vWF) in lung sections
- Anesthesia of rat pups
- Lung tissue harvesting and preparation for immunohistochemistry and histological analyses
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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