Protocol detail

Immunohistochemical Detection of FHL2 in Ovarian Tissues

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A previously described peroxidase-based immunohistochemistry protocol was used to detect FHL2 expression in paraffin-embedded ovarian tissues [36 (link)]. Anti-FHL2 antibody (Proteintech, Wuhan, China) was used at 1:200. Subsequently, the sections were incubated with biotinylated secondary antibody (1:2000) (Boster Biotechnology, Wuhan, China) and avidin-biotin-peroxidase (Boster Biotechnology, Wuhan, China) before being exposed to diaminobenzidine (Servicebio, Wuhan, China, Wuhan, China) and counterstained with hematoxylin (Servicebio, Wuhan, China).

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Wang C., Sun H., Davis J.S., Wang X., Huo L., Sun N., Huang Q., Lv X., Wang C., He C., He C., Zhou Y., Wu J., Yang L, & Hua G. (2023). FHL2 deficiency impairs follicular development and fertility by attenuating EGF/EGFR/YAP signaling in ovarian granulosa cells. Cell Death & Disease, 14(4), 239.

Publication 2023

biotinylated secondary antibody avidin-biotin-peroxidase diaminobenzidine hematoxylin

Anti antibody Antibody Avidin Biotin Embedded paraffin Fhl2 Hematoxylin Immunohistochemistry Ovarian Peroxidase Tissues

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Variable analysis

independent variables

Anti-FHL2 antibody concentration (1:200)

dependent variables

FHL2 expression in paraffin-embedded ovarian tissues

control variables

Paraffin-embedded ovarian tissues

controls

No positive or negative controls were explicitly mentioned in the provided information.

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