Antioxidant Efficacy of MBGCe_Gen

The antioxidant property of MBGCe_Gens was estimated as their ability to remove H₂O₂, one of the most significant ROS species. In correlation with the role of enzyme Catalase, the property is named CAT activity. The tests were performed using the Fluorimetric Hydrogen Peroxide Assay Kit from Sigma Aldrich with a TECAN GeniosPro microplate reader, as presented in a previous paper [16 (link)] Again, measurements were performed in SBF to simulate the physiological environment surrounding the implant. The presence of H₂O₂ in SBF is detected through its reaction with a molecular probe catalyzed by the peroxidase enzyme, which generates a red fluorescent product that can be analyzed fluorometrically. CAT activity is reported as the percentage of H₂O₂ decomposed at the end of the assay. We suspended 40 mg of MBGCe_Gen in 400 μL of 50 μM solution of H₂O₂ in SBF and measured the residual concentration of H₂O₂ after 120 min of soaking.