Feeder-Independent hESC and hiPSC Culture

The feeder-independent human embryonic stem cell (hESC) line, WA09, was obtained from the WiCell mTeSR™1 Bank and cultivated in either mTeSR™1 (Stem Cell Technologies, 05850) or adapted to StemPro® hESC SFM (Life Technologies, A1000701). Cell stocks were continuously maintained in feeder-independent conditions on hESC-Qualified Matrigel™ (Becton Dickinson, 354277) in a humidified, 37°C cell incubator equilibrated with 5% CO₂. Later, an additional feeder-independent hESC line, WA07 (WiCell mTeSR™1 Bank) and two human induced pluripotent stem cell (hiPSC) lines, hiPSC18R and hiPSC19K (Lonza–Walkersville), were also cultivated continuously in the hypertonic citrate solution to determine if additional hPSC lines could also maintain their pluripotency. Both hiPSC lines were generated from human cord blood CD34+ cells.

Free full text: Click here

Nie Y., Walsh P., Clarke D.L., Rowley J.A, & Fellner T. (2014). Scalable Passaging of Adherent Human Pluripotent Stem Cells. PLoS ONE, 9(1), e88012.

Publication 2014

mTeSR™1 StemPro® hESC SFM hESC-Qualified Matrigel™

Blood cells Cell lines Cells Citrate Cord Cord blood Human Human embryonic stem cell Human induced pluripotent stem cell Hypertonic solution Matrigel Stem Stem cell

Corresponding Organization :

Other organizations : Lonza (United States)

Top 5 similar protocols

Protocol cited in 6 other protocols

Variable analysis

independent variables

Cultivation of the hESC line WA09 in either mTeSR™1 or StemPro® hESC SFM
Cultivation of the additional hESC line WA07 and hiPSC lines hiPSC18R and hiPSC19K in the hypertonic citrate solution

dependent variables

Maintenance of pluripotency in the hESC and hiPSC lines

control variables

Cultivation of all cell lines on hESC-Qualified Matrigel™ in a humidified, 37°C cell incubator equilibrated with 5% CO2

controls

Positive control: Cultivation of the hESC line WA09 in mTeSR™1
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!