Cell populations were isolated by sorting following flow cytometry with fluorochrome-conjugated antibodies. Cells were prepared as described above and all incubation and washing steps performed in 1% fetal calf serum in Dulbecco’s Modified Eagle Medium (DMEM). After a final wash prior to sorting, cells were filtered through a 70-μm nylon cell strainer (Becton Dickinson) for removal of cell clumps. Cell sorting was performed using a FACSAria cell sorter (Becton Dickinson). Sorted populations were collected in complete medium (10% fetal calf serum in DMEM) as described previously (32 (link)).
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