Western blots of FoxP3 were performed as described before27 (link); intensity of FoxP3 bands detected with anti-FoxP3 antibody (eBio7979, eBioscience) was analyzed using ImageJ 1.48 v (NIH) software and values are calculated relative to the corresponding intensity of heat shock protein 70 (HSP70) bands detected with anti-HSP70 antibody (1B5, Assay designs).
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