The following antibodies were used: mouse monoclonal antibodies to β gal
(1:1,000, G8021, Sigma), pERK (1:50, sc-7383, Santa Cruz), CC1 (1:50, OP80,
Millipore), neurofilament (1:1,000, NE1017, Millipore), and rabbit polyclonal
antibodies to Olig2 (1:500, AB9610, Millipore), ki67 (1:500, SP6, Cell Marque),
Caspr (1:1,000) (ref. 68 (link)), pERK (1:1,000, 4370P,
Cell Signaling), ERK (1:2,000, M5670, Sigma), GPR17 (1:20, Cayman Chemical,
17087), and goat polyclonal antibody to GPR37 (1:50, Santa Cruz, sc-27548), and
rat monoclonal antibodies to MBP (1:300, MAB386, Chemicon), PDGFRα
(1:1,000, APA5, BD Pharmingen), and hybridoma supernatants of mouse anti-O4
(1:5) and rat anti-PLP (1:10, AA3). Secondary antibodies were obtained from
Jackson Immunoresearch and Invitrogen. PLX4032 (ref. 41 (link)) and myr-EPE (ref. 40 (link)) are
generous gifts from Prof. Rony Seger (Weizmann Institute of Science, Rehovot,
Israel). SQ 22536, IBMX and ESI-09 were purchased from Sigma. cAMP levels were
determined by ELISA according to the manufacturer's protocol (cAMP
ELISA kit, Enzo Life Sciences).
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