For chondrogenic differentiation, cells were cultured as cell aggregates on ultra-low attachment plates (Corning), as previously reported [11 (link)]. Cells were maintained in a chondrogenic differentiation medium (MesenCult™ Chondrogenic Differentiation Kit, Stemcell Technologies, Vancouver, BC, Canada) for 21 days, and chondrogenic differentiation was confirmed with Alcian Blue staining (Sigma-Aldrich) [11 (link)].
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