Example 13

Solution 1 (2% of perillaldehyde), Solution 2 (0.008 μg/mosquito of dinotefuran), and Solution 3 (0.008 μg/mosquito of dinotefuran with 2% of perillaldehyde) were tested for efficacy against 3- to 5-day old adult Culex quinquefasciatus. At 1 hour we obtained 33%, 10%, and 100% knockdown for Solutions 1, 2, and 3, respectively. At 24 hours we obtained 13%, 77%, and 100% mortality for Solutions 1, 2, and 3, respectively. The CO2 control and acetone standard both had 0% knockdown at 1 hour, and 0% mortality at 24 hours. Results are shown in Table 5.

TABLE 5
Efficacy of perillaldehyde, dinotefuran, and a combination of both against adult, virgin,
female Culex quinquefasciatus mosquitoes.
ACTIVE INGREDIENTCONCENTRATION% MORTALITY AFTER 24 HRS
PERILLALDEHYDE2%13
DINOTEFURAN0.008 μg77
OBS.*CALC.**
PERILLALDEHYDE + DINOTEFURAN2% + 0.008 μg10079.99
*Obs. = observed efficacy
**Calc. = efficacy calculated using Colby (1967) formula
Since the actual insecticidal kill rate exceeds the calculated value, then the action of the combination is super-additive or a synergistic effect is present.

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