Transmission Electron Microscopy of Cell Migration
To further analyze PMN and T-cells migrating across the HIBCPP cells, we prepared filter membranes from different experimental conditions for transmission electron microscopy (HIBCPP + PMN + T-cells ± chemokine ± E-30 Bastianni) as described previously [39 (link)]. Briefly, after 28 h of infection and subsequent leukocyte migration, the filter inserts were fixed over night at 4 °C in 2% glutaraldehyde/0.1 M cacodylate buffer (pH 7.4) embedded in Araldite (Science Services, Germany) and ultrathin sections were performed using an ultramicrotome (Ultracut R) (Leica, Germany). Samples were analyzed with a Zeiss Em 10 Transmission electron microscope (Zeiss, Germany).
Other organizations :
Heidelberg University, University Hospital Heidelberg, Heinrich Heine University Düsseldorf, Düsseldorf University Hospital, Robert Koch Institute, University Children's Hospital Tübingen, Carl Zeiss (Germany), Natural and Medical Sciences Institute, University of Tübingen, University Medical Centre Mannheim, Nippon Dental University
Morphology and migration of PMN and T-cells across HIBCPP cells
control variables
Infection duration (28 h)
Fixation conditions (2% glutaraldehyde/0.1 M cacodylate buffer, pH 7.4)
Embedding medium (Araldite)
Microscopy technique (Transmission electron microscopy)
controls
Not explicitly mentioned
Not explicitly mentioned
Annotations
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