The cDNA was synthesized via reverse transcription using the PrimeScriptTM RT reagent Kit with gDNA Eraser (TaKaRa). Then, the qRT-PCR was performed using TB Green® Premix Ex Taq™ II (Tli RNaseH Plus) (TaKaRa) on an ABI ViiA 7 system. The results were analyzed using the relative quantitative method, and the mRNA expression of genes was normalized with Gapdh. The primers for qRT-PCR are shown in Table S1 .
A strand-specific reverse transcriptase reaction was performed using the method a previous study reported [67 (link)] for the detection of paternally expressed Rtl1 using the PrimeScriptTM RT reagent Kit with gDNA Eraser (TaKaRa). The PCR results were quantified with Image J 1.53e software (Wayne Rasband and contributors National Institutes of Health, USA,http://imagej.nih.gov/ij ), and the results were normalized to Gapdh expression.
A strand-specific reverse transcriptase reaction was performed using the method a previous study reported [67 (link)] for the detection of paternally expressed Rtl1 using the PrimeScriptTM RT reagent Kit with gDNA Eraser (TaKaRa). The PCR results were quantified with Image J 1.53e software (Wayne Rasband and contributors National Institutes of Health, USA,
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