HS versus Phx cytokine levels were analyzed with a Luminex bead array platform (Life Technologies) according to the manufacturer’s instructions. All samples were analyzed in triplicate and compared against multiple internal standards with a nine-point standard curve. Data were acquired on a FlexMAP-3D system (Luminex), and analysis was performed in XPonent 4.0 software (Luminex), as well as through five-parameter logistic regression.32 (link)
Ghassemi S., Martinez-Becerra F.J., Master A.M., Richman S.A., Heo D., Leferovich J., Tu Y., García-Cañaveras J.C., Ayari A., Lu Y., Wang A., Rabinowitz J.D., Milone M.C., June C.H, & O’Connor R.S. (2020). Enhancing Chimeric Antigen Receptor T Cell Anti-tumor Function through Advanced Media Design. Molecular Therapy. Methods & Clinical Development, 18, 595-606.
Multiple internal standards with a nine-point standard curve
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