Immunocytochemical Detection of OCT4 and TROP2

Immunocytochemistry was performed as described before 34 (link) using anti-OCT4 (sc-5279, Santa Cruz Biotechnology) and anti-TROP2 (BD Bioscience) antibodies. Cell nuclei were counterstained with DAPI.

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Li E., Zhang Z., Jiang B., Yan L., Park J.W, & Xu R.H. (2018). Generation of Mesenchymal Stem Cells from Human Embryonic Stem Cells in a Complete Serum-free Condition. International Journal of Biological Sciences, 14(13), 1901-1909.

Publication 2018

anti-OCT4 (sc-5279, anti-TROP2

Antibodies Cell nuclei Dapi Immunocytochemistry Oct4

Corresponding Organization : University of Macau

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Variable analysis

independent variables

Anti-OCT4 (sc-5279, Santa Cruz Biotechnology) antibody
Anti-TROP2 (BD Bioscience) antibody

dependent variables

Expression of OCT4 protein
Expression of TROP2 protein

control variables

Cell nuclei stained with DAPI

controls

No positive or negative controls were explicitly mentioned.

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