Western Blot Analysis of Cellular Signaling

Cell lysates were prepared and Western blot analysis was performed as previously described [19 , 40 (link)]. Following antibodies were used: anti-G6PD antibody (ab133525, Abcam, Cambridge, U.K.); anti-STAT3 antibody (4904, Cell Signaling Technology), anti-phospho-STAT3 antibody (Ser 727) (ab30647), anti-CyclinD1 antibody (ab16663, Abcam), Anti-CDK4 antibody (ab108357), Anti-p105/50 antibody (3035, Cell Signaling Technology), anti-p65 antibody (ab32536), anti-pIκBα (Ser32 + Ser36) antibody (AF2002, Affinity, USA), anti-IκBα antibody (AF5002, Affinity, USA), anti-β-actin antibody (4967, Cell Signaling Technology), and anti-GAPDH antibody (2118, Cell Signaling Technology).

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Zhang Q., Yang Z., Ni Y., Bai H., Han Q., Yi Z., Yi X., Agbana Y.L., Kuang Y, & Zhu Y. (2020). NF-κB and pSTAT3 synergistically drive G6PD overexpression and facilitate sensitivity to G6PD inhibition in ccRCC. Cancer Cell International, 20, 483.

Publication 2020

ab133525 ab30647 ab16663 AF2002 AF5002 anti-β-actin antibody anti-GAPDH antibody

Actin Anti antibody Antibodies Antibody Cell G6pd Gapdh Iκbα Stat3 Western blot analysis

Corresponding Organization : First Affiliated Hospital of Kunming Medical University

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

STAT3
Phospho-STAT3 (Ser 727)
CyclinD1
P105/50
PIκBα (Ser32 + Ser36)
IκBα
β-actin
GAPDH

control variables

Not explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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