Proteins were collected from each sample and analyzed according to the methods described previously19 (link),28 (link). BTG2, CREBH, SHP, pAKT, total AKT, β-actin (Santa Cruz Biotechnology, Santa Cruz, CA, USA), CRBN, pAMPK, AMPK, pS6K, S6K (Cell Signaling Technology, Danvers, MA, USA), and SESN2 (Protein Tech, Chicago, IL, USA) antibodies were used as described previously9 (link),14 (link),19 (link),27 (link)–30 (link).
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