Quantifying Biofilm Cellulose Structure

The cellulose of the biofilm on the coverslips was washed with PBS three times to remove the planktonic bacteria. The coverslips were then placed at 60 °C for 1 h to fix the biofilm and then stained with 0.1 mg/mL calcofluor white for 20 min in a dark room. After being washed with PBS three times, the biofilm was observed with a fluorescence microscope (SUNNY ICX41, Yuyao, China).

Free full text: Click here

Zhang J., Liu Y., Hu J., Leng G., Liu X., Cui Z., Wang W., Ma Y, & Sha S. (2024). Cellulase Promotes Mycobacterial Biofilm Dispersal in Response to a Decrease in the Bacterial Metabolite Gamma-Aminobutyric Acid. International Journal of Molecular Sciences, 25(2), 1051.

Publication 2024

Corresponding Organization : Dalian Medical University

Top 5 similar protocols

Variable analysis

independent variables

Duration of biofilm fixation (1 h)

dependent variables

Presence and distribution of cellulose in the biofilm

control variables

Washing the biofilm with PBS three times
Staining the biofilm with 0.1 mg/mL calcofluor white for 20 min
Temperature of biofilm fixation (60 °C)

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!