Primary cultured cells were transfected with dominant negative STAT3(S727A), constitutively active STAT3(S727E), dominant negative STAT3(Y705F) or wild type STAT3 as control (kind gift of Dr Too, National University of Singapore, Singapore (32 (link))). Transfection was achieved using X-treme transfection reagent (Roche, MA) in serum free medium for 24 hours.
Protocol detail
STAT3 Signaling Pathway Modulation
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Corresponding Organization : Virginia Commonwealth University Medical Center
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Variable analysis
independent variables
- Dominant negative STAT3(S727A)
- Constitutively active STAT3(S727E)
- Dominant negative STAT3(Y705F)
- Wild type STAT3 as control
- Not explicitly mentioned
- Transfection was achieved using X-treme transfection reagent (Roche, MA) in serum free medium for 24 hours.
- Wild type STAT3 as control
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