The animals were anesthetized with an intraperitoneal injection of ketamine 100 mg/kg (Fort Dodge Animal Health, Fort Dodge, Iowa) and Xylazine 6 mg/kg (Phoenix Scientific, Inc., St. Joseph, MO). The glottis was directly visualized and intubated with a 18 gauge Angiocatheter (forced oscillation measurements)(BD Insyte, Sandy, Utah) and transferred to a FlexiVent rodent ventilator (Scireq, Montreal, QC Canada) as previously described [12 (link)]. Ventilator rates of 200/minute, tidal volume of10 ml/kg with positive end-expiratory pressures of 3 cmH2O and a pressure limit of 30 cmH2O were used for the pneumonectomy procedure.
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Rodent Anesthesia and Ventilation for Pneumonectomy
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Corresponding Organization : Harvard University
Other organizations : University Medical Center of the Johannes Gutenberg University Mainz, Johannes Gutenberg University Mainz
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Variable analysis
independent variables
- Intraperitoneal injection of ketamine 100 mg/kg
- Intraperitoneal injection of Xylazine 6 mg/kg
- Forced oscillation measurements
- Ventilator rates of 200/minute
- Tidal volume of 10 ml/kg
- Positive end-expiratory pressures of 3 cmH2O
- Pressure limit of 30 cmH2O
- Positive control: Not specified
- Negative control: Not specified
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