Planarian and 293T Luminescence Imaging

The Leica DMi8 microscopy and Andor iKon-M 934 CCD camera were used to take pictures of luminescence from planarian cells and 293T cells with a 20x air objective (Leica, 506521). The 200,000 planarian live cells were resuspended in 50 μL culture medium and transferred to a glass-bottom dish (NEST, 801002) for imaging. The luminescence was measured after directly adding 1 μL Nano-Glo luciferase assay substrate (Promega, N1110) to cells. The exposure time was set to 20 s for luminescence imaging, and 1 s for bright field imaging.

Free full text: Click here

Lei K., Zhang W., Chen J., McKinney S.A., Ross E.J., Lee H.C, & Sánchez Alvarado A. (2023). Pluripotency retention and exogenous mRNA introduction in planarian stem cells in culture. iScience, 26(2), 106001.

Publication 2023

iKon-M 934 CCD camera Nano-Glo luciferase assay substrate

293t cells Assay Cells Culture medium Dish Luciferase Luminescence Microscopy Planarian Promega

Corresponding Organization : Howard Hughes Medical Institute

Other organizations : University of Chicago

Top 5 similar protocols

Variable analysis

independent variables

Number of planarian live cells (200,000)
Amount of Nano-Glo luciferase assay substrate (1 μL)

dependent variables

Luminescence from planarian cells
Luminescence from 293T cells

control variables

Microscopy equipment (Leica DMi8 microscope and Andor iKon-M 934 CCD camera)
Objective lens (20x air objective, Leica 506521)
Cell culture medium
Glass-bottom dish (NEST, 801002)
Exposure time for luminescence imaging (20 s)
Exposure time for bright field imaging (1 s)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!