Halogenated Flame Retardant Analysis

Extracts in hexane were analyzed for halogenated
flame retardants (Br/Cl-FRs), including fifty-one PBDEs, using gas
chromatography negative ion mass spectrometry (GC-ECNI-MS) (Table S1). The Br/Cl-FRs were determined using
HP-5MS (Agilent Technologies, Inc., U.S.A.) or RTX-1614 (Restek Corporation,
U.S.A.) capillary columns. Co-eluting congeners, including BDE-17/25,
BDE-28/33, and BDE-138/166 were quantified together. PBDEs were monitored
at m/z 79 and 81 and quantified
using external standard calibration. Other Br/Cl-FRs were monitored
by characteristic fragments (Table S1).
Gas chromatographic oven temperature and mass spectral conditions
are given in Table S2.