Targeted Biogenic Amine Metabolomics

Four ~1.5-g frozen tissue samples collected from specimens 25, 27, 28, and 29 each in 1:1 wt:wt sample to EtOH ratio solutions were shipped on dry ice for biogenic amines panel metabolome analyses by HILIC-QTOF MS/MS [76 (link)] at the UC Davis West Coast Metabolomic Center (http://metabolomics.ucdavis.edu/). Raw output files were curated based on internal standard removal, signal to noise ratio cutoff, and a minimum peak threshold of 1000 and subsequently parsed based on average (n=4) metabolite log fold increases relative to blanks, as suggested elsewhere [77 (link)]. Only metabolites with non-redundant names and InChIKey identifiers and average fold changes higher than 5 relative to blanks were retained for further analyses.

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Ramírez G.A., Bar-Shalom R., Furlan A., Romeo R., Gavagnin M., Calabrese G., Garber A.I, & Steindler L. (2023). Bacterial aerobic methane cycling by the marine sponge-associated microbiome. Microbiome, 11, 49.

Publication 2023

Biogenic amines Dry ice Etoh Frozen Metabolome Ms ms Tissue

Corresponding Organization :

Other organizations : University of Haifa, Carmel (Israel), National Institute of Oceanography and Applied Geophysics, Arizona State University

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Variable analysis

independent variables

Tissue samples collected from specimens 25, 27, 28, and 29

dependent variables

Biogenic amines panel metabolome

control variables

1:1 wt:wt sample to EtOH ratio solutions
Shipped on dry ice
Signal to noise ratio cutoff
Minimum peak threshold of 1000
Average (n=4) metabolite log fold increases relative to blanks

controls

Blanks

Annotations

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