Synthesis and Evaluation of Multifunctional Nanoparticles
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : Wuhan University of Technology
Variable analysis
- Concentration of 3,3′,5,5′-Tetramethylbenzidine (TMB)
- Concentration of β-D-Glucose (β-D-Glu)
- Concentration of Ferric chloride hexahydrate (FeCl3·6H2O)
- Concentration of Ferrous chloride tetrahydrate (FeCl2·4H2O)
- Concentration of Oleic acid (OA)
- Concentration of Pluronic F127
- Concentration of PLGA (50:50)
- Concentration of Glucose oxidase (GOx)
- Cell viability/cytotoxicity (measured by CCK8 assay and Calcein-AM/PI staining)
- Reactive oxygen species (ROS) generation (measured by DMPO spin trapping)
- Cell line (HeLa cells)
- Culture media (DMEM, high-glucose DMEM)
- Serum (fetal bovine serum albumin, FBSA)
- Buffers and reagents (PBS, trypsin)
- Analytical techniques (CCK8 assay, Calcein-AM/PI staining, DMPO spin trapping)
- No positive controls were explicitly mentioned.
- No negative controls were explicitly mentioned.
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!