RT-qPCR was performed to detect miRNA and mRNA as mentioned above [20 (link)]. The extraction of total RNA was from NPC tissues and cells adopting TRIzol kit (Invitrogen). Then, RNA was reverse transcribed into a complementary DNA (PrimeScript RT Master Mix, Takara, Dalian, China), and amplification was on a real-time PCR instrument. The PCR was accomplished in the light of the instructions in the SYBR Green RT-qPCR kit (Takara). U6 and β-actin were adopted as loading control for miRNA and mRNA, respectively. The adoption of 2−ΔΔCT was to normalize the gene. Primer sequences are presented in Table 1 .
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