Matrigel Invasion Assay for Pancreatic Cancer Cell Motility

The motility of the pancreatic cancer cell lines was assessed by Matrigel invasion assay as previously described 20 (link). Briefly, siRNA-transfected cells (2.0×10⁵) were placed in the upper chamber of a Transwell chamber and incubated for 18 h at normoxic conditions. The cells that invaded the lower chamber were fixed and stained with Diff-Quik reagents following the manufacturer's protocol (Sysmex Corporation). The stained cells were counted at an ×200 magnification under a light microscope (Nikon Eclipse TE 300; Nikon Corporation).

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Nagao S., Onishi H., Kawamoto M., Masuda S., Na L., Morisaki S., Iwamoto N., Yamada Y., Koga S., Ichimiya S., Nakayama K., Imaizumi A., Nakashima K., Oda Y, & Nakamura M. (2023). C4orf47 contributes to the dormancy of pancreatic cancer under hypoxic conditions. Journal of Cancer, 14(2), 306-317.

Publication 2023

Diff-Quik reagents Nikon Eclipse TE 300

Assay Cancer of the pancreatic Cell lines Cells Light microscope Matrigel Motility Sirna

Corresponding Organization :

Other organizations : Kyushu University

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Variable analysis

independent variables

SiRNA transfection

dependent variables

Matrigel invasion (cell motility)

control variables

Incubation time (18 h)
Incubation conditions (normoxic)
Cell seeding density (2.0×10^5 cells)
Staining method (Diff-Quik reagents)
Microscope magnification (×200)

controls

No controls were explicitly mentioned in the provided information.

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