Agar plugs (6 mm diam) were taken from the edge of actively growing cultures on MEA and transferred onto the centre of 9 cm diam Petri dishes containing 2 % tap water agar supplemented with sterile pine needles (PNA; Smith et al. 1996 ), potato dextrose agar (PDA), oatmeal agar (OA) and MEA (Crous et al. 2009 ), and incubated at 20–21 °C under a 12 h near-ultraviolet light/12 h dark cycle to induce sporulation as described in recent studies (Gomes et al. 2013 (link), Lombard et al. 2014 ). Colony characters and pigment production on MEA, OA and PDA were noted after 10 d. Colony colours were rated according to Rayner (1970) . Cultures were examined periodically for the development of ascomata and conidiomata. Colony diameters were measured after 7 and 10 d. The morphological characteristics were examined by mounting fungal structures in clear lactic acid and 30 measurements at ×1000 magnification were determined for each isolate using a Zeiss Axioscope 2 microscope with interference contrast (DIC) optics. Descriptions, nomenclature and illustrations of taxonomic novelties are deposited in MycoBank (www.MycoBank.org;Crous et al. 2004b ).