Cell Traction Quantification Protocol

We followed the protocols of cell-traction quantification published previously11 (link). In brief, cells were plated on PA gels with red fluorescent beads (0.2 μm in diameter; Molecular Probes, Invitrogen) embedded on the top surface. Before and after cell trypsinization, fluorescent images were taken to compute the displacement field of the beads using digital image correlation (DIC)in a homebuilt MATLAB program. Cell tractions were then calculated from the displacement field using inverse Boussinesq mathematical model.

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Tan Y., Tajik A., Chen J., Jia Q., Chowdhury F., Wang L., Chen J., Zhang S., Hong Y., Yi H., Wu D.C., Zhang Y., Wei F., Poh Y.C., Seong J., Singh R., Lin L.J., Doğanay S., Li Y., Jia H., Ha T., Wang Y., Huang B, & Wang N. (2014). Matrix softness regulates plasticity of tumour-repopulating cells via H3K9 demethylation and Sox2 expression. Nature Communications, 5, 4619.

Publication 2014

red fluorescent beads

Cell Gels Molecular probes Tractions

Corresponding Organization :

Other organizations : Huazhong University of Science and Technology, University of Illinois Urbana-Champaign, La Jolla Bioengineering Institute, University of California, San Diego

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Variable analysis

independent variables

Cells plated on PA gels with red fluorescent beads

dependent variables

Displacement field of the beads calculated using digital image correlation (DIC)
Cell tractions calculated from the displacement field using inverse Boussinesq mathematical model

control variables

Diameter of the red fluorescent beads (0.2 μm)
Homebuilt MATLAB program used for DIC

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