General DNA manipulations were performed using standard procedures. DNA samples were heated at 75°C for 10 min prior to the electrophoresis to ensure cos site melting. The plasmids and oligonucleotides used in this study are listed in the electronic supplementary material, tables S2 and S3, respectively. The labelling of the probes and DNA hybridization were performed according to the protocol supplied with the PCR-DIG DNA-labelling and Chemiluminescent Detection Kit (Roche). To produce the phage and SaPI mutations, we used plasmid pBT2-βgal, as previously described [11 (link)].
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