Adoptive T cell transfer protocols

After purification, T cells were labeled with 5 μM of either CFSE (Invitrogen) or CellTrace^TM Violet (Molecular Probes), as previously described (26 (link)) and injected i.v. into hosts. For inducing lymphopenia, normal B6 mice were treated with anti-Thy1.2 mAb 30-H12 (anti-Thy1.2) (Bio X Cell, i.p. injection in a single dose of 200 μg/mouse, 2 days before cell transfer) or 600cGy of whole-body irradiation (1 day before cell transfer). For generating antigen-induced “SP-like” response, SMARTA CD4⁺ T cells were transferred into the hosts, as indicated in the figures, followed by either LCMV Armstrong (2 × 10⁵ PFU) or LCMV peptide GP₆₁₋₈₀ (20 μg/mouse) through i.p. injection 1 day post cell transfer. OT-I CD8⁺ T cells were transferred into the hosts, as indicated in the figures, followed by immunization of OVA protein (Sigma Aldrich, 100 μg/mouse). HY.CD8⁺ cells from female HY mice were transferred into female hosts.