Cytogenetic studies using conventional G‐banding were performed on BM samples. At least 20 metaphases were analyzed whenever possible. Karyotypes were recorded according to the International System for Human Cytogenetic Nomenclature (ISCN) 2013.18 Interphase fluorescence in situ hybridization (FISH) analysis was performed on mononuclear cells of BM aspirates to detect frequent abnormalities such as 5/5q‐, −7, −7q‐, +8, −20/20q‐, and +1/1q + using Vysis LSI EGR1 (5q31), D7S522 (7q31), CEP8, Trisomy 1q (1q25), and D20S108 (20q12) probes (Abott Downers).
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Cytogenetic Analysis of Bone Marrow
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Abnormalities
Cells
Egr1
Fluorescence in situ hybridization
Human
Interphase
Karyotypes
Metaphases
Trisomy
Corresponding Organization : Seoul National University
Other organizations : Chungbuk National University Hospital, Chungbuk National University, Korea University, Seoul National University Hospital
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Variable analysis
independent variables
- Cytogenetic studies using conventional G‐banding on BM samples
- Karyotypes recorded according to the International System for Human Cytogenetic Nomenclature (ISCN) 2013
- Interphase fluorescence in situ hybridization (FISH) analysis on mononuclear cells of BM aspirates to detect frequent abnormalities such as 5/5q‐, −7, −7q‐, +8, −20/20q‐, and +1/1q +
- At least 20 metaphases were analyzed whenever possible
- Vysis LSI EGR1 (5q31), D7S522 (7q31), CEP8, Trisomy 1q (1q25), and D20S108 (20q12) probes (Abott Downers)
- Not explicitly mentioned
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