Optical self-stimulation for positive reinforcement

For positive reinforcement induced by vGAT-containing BNST-PBN projections, mice with optical fibers implanted above the PBN were trained in one 1-hour session to nose poke on a fixed-ratio 1 schedule for optical self-stimulation (3 s, 20 Hz, ~10 mW, 473 nm) in a mouse operant chamber (17.8 cm by 15.2 cm by 18.4 cm; Med Associates) (20 (link)). The following day, the mice were run again with the same conditions, and the number of nose pokes recorded in the hour session was recorded.

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Luskin A.T., Bhatti D.L., Mulvey B., Pedersen C.E., Girven K.S., Oden-Brunson H., Kimbell K., Blackburn T., Sawyer A., Gereau RW I.V., Dougherty J.D, & Bruchas M.R. (2021). Extended amygdala-parabrachial circuits alter threat assessment and regulate feeding. Science Advances, 7(9), eabd3666.

Publication 2021

mouse operant chamber

Mice Nose Pokes Positive reinforcement Self stimulation

Corresponding Organization : University of Washington

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Variable analysis

independent variables

Optical stimulation of vGAT-containing BNST-PBN projections

dependent variables

Number of nose pokes during the 1-hour session

control variables

Mouse operant chamber size (17.8 cm by 15.2 cm by 18.4 cm)
Optical stimulation parameters (3 s, 20 Hz, ~10 mW, 473 nm)
Fixed-ratio 1 schedule for optical self-stimulation

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