Annexin V-FITC Apoptosis Detection

Apoptosis was measured as previously described (19 (link)) using the annexin V-fluorescein isothiocyanate (annexin V-FITC) Apoptosis Detection Kit (BD Biosciences, San Diego, CA) according to the manufacturer’s instructions. Following treatment, annexin positive cells were quantitated by FACS analysis (Becton Dickinson, Ranklin Lakes, NJ) using FlowJo Software (Tree Star, Inc, Ashland, OR) and FCS Express 7 Software (De Novo Software Inc.). The percentage of annexin positive was calculated and the delta between treated and untreated control was determined.

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Safari M., Litman T., Robey R.W., Aguilera A., Chakraborty A.R., Reinhold W.C., Basseville A., Petrukhin L., Scotto L., O'Connor O.A., Pommier Y., Fojo A.T, & Bates S.E. (2021). R-Loop–Mediated ssDNA Breaks Accumulate Following Short-Term Exposure to the HDAC Inhibitor Romidepsin. Molecular Cancer Research, 19(8), 1361-1374.

Publication 2021

annexin V-fluorescein isothiocyanate (annexin V-FITC) Apoptosis Detection Kit

Annexin Annexin v Apoptosis Cells Fluorescein Isothiocyanate Tree

Corresponding Organization : Columbia University

Other organizations : University of Copenhagen, National Cancer Institute, Center for Cancer Research, Centro Andaluz de Biología Molecular y Medicina Regenerativa, Universidad Pablo de Olavide, Universidad de Sevilla, Institut de Cancérologie de l'Ouest, University of Virginia

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Variable analysis

independent variables

Treatment

dependent variables

Apoptosis
Percentage of annexin positive cells

control variables

Untreated control

controls

Positive control: Not specified
Negative control: Untreated control

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