Mice were sacrificed and brain sections were prepared as previously reported (Deng, 2013 (link)). Primary antibodies used were: rat anti-BrdU (1:250, AbD Serotec CAT# OBT0030CX), mouse anti-NeuN (1:100, Millipore (CHEMICON/Upstate/Linco) CAT# MAB377), and goat anti-DCX (1:250, Santa Cruz Biotechnology CAT# sc-8066). All secondary antibodies were from Jackson ImmunoResearch (Donkey anti-rat IgG Cy3 CAT# 712-165-153, donkey anti-mouse IgG Alexa Fluor 647 CAT# 715-605-151, donkey anti-goat IgG Alexa Fluor 488 CAT# 705-545-147) at 1:250 dilutions. DAPI (Sigma–Aldrich CAT# D9542) was used to stain individual cells.
Protocol detail
Immunohistochemical Characterization of Neural Markers
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OBT0030CX
goat anti-DCX
Donkey anti-rat IgG Cy3
donkey anti-mouse IgG Alexa Fluor 647
donkey anti-goat IgG Alexa Fluor 488
DAPI
Alexa fluor 488
Alexa fluor 647
Anti igg
Antibodies
Brain
Brdu
Cells
Dapi
Dilutions
Donkey
Goat
Mouse
Stain
Corresponding Organization : Salk Institute for Biological Studies
Other organizations : UCLA Health, University of California, Los Angeles
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Variable analysis
independent variables
- None explicitly mentioned
- Brain sections
- Levels of BrdU, NeuN, and DCX proteins
- None explicitly mentioned
- Positive control: Not specified
- Negative control: Not specified
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