The capacity of probiotic Lactobacillus strains to assimilate cholesterol in MRS broth was investigated as previously described [18 (link)]. Cholesterol-PEG 600 (Sigma Aldrich, St. Louis, MI, USA) was added to MRS broth at a final concentration of 100 μg/mL. The 1% (v/v) inoculum of each overnight probiotic culture was added and incubated at 37 °C for 24 h. The bacterial cultures were centrifuged at 4000 rpm for 10 min at 4 °C, and the supernatants with non- assimilated cholesterol were collected [24 (link)]. Cholesterol assimilation was calculated by the formula:
where A is the cholesterol that remained with the pellet (as a percentage), B is the absorbance of the sample containing the cells, and C is the absorbance of the sample without bacterial cells. The isolates with the highest cholesterol assimilating potential were selected for in vivo trials. The Lactobacillus plantarum ATCC 14917 (KWIK-STIK, UK) was used as a reference strain only for cholesterol assimilation activity.
where A is the cholesterol that remained with the pellet (as a percentage), B is the absorbance of the sample containing the cells, and C is the absorbance of the sample without bacterial cells. The isolates with the highest cholesterol assimilating potential were selected for in vivo trials. The Lactobacillus plantarum ATCC 14917 (KWIK-STIK, UK) was used as a reference strain only for cholesterol assimilation activity.
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