Optimized Mating Receptor Expression

As detailed in table S1, some mating receptor ORFs were synthesized as codon-optimized genes for S. cerevisiae (see table S6), and others were cloned directly from the appropriate fungal genomic DNA (ATCC) or plasmid pLPreB using primers in table S5. Codon optimization was performed with the JCat Codon Adaptation tool (38 (link)) using the default setting for S. cerevisiae and further optimized for cloning with the IDT codon optimization tool (Integrated DNA Technologies). All receptor ORFs were incorporated into expression modules containing the S. cerevisiae TDH3 promoter and STE2 terminator (see table S4). For fluorescent assays using reporter strain yMJ183, receptor expression modules were cloned into low-copy plasmids derived from pRS416 (table S3). For lycopene biosensor strains, receptor expression modules were integrated at the STE2 locus.