Samples were collected from the first and second leaves of ten plants for each treatment. Leaves were weighed 0.2 g (fresh weight, FW). The tea leaves were ground with 10 mL of 80% acetone, the homogenate was filtered, and then the samples were centrifuged at 4000×g for 10 min to collect the supernatant. The optical density was measured with a U-5100 spectrophotometer (Hitachi, Japan) at 663 nm (OD663) and at 645 nm (OD645) for chlorophyll a (Chl a) and chlorophyll b (Chl b), respectively, using the following formulae: Chla concentrations = 12.72 × A663 − 2.59 × A645, Chlb concentrations = 22.88 × A649 − 4.67×A663.
where V and FW indicate the volume of the reaction system and the weight of the isolated tea leaves, respectively [41 (link)].
where V and FW indicate the volume of the reaction system and the weight of the isolated tea leaves, respectively [41 (link)].
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