Multi-parameter Flow Cytometry Protocol

Staining of surface markers was performed as described earlier [42 (link)]. The following anti-human monoclonal antibodies were used: phycoerythrin (PE)-conjugated CD33 (MACS Milteyni Biotec, Leiden, The Netherlands); PE-Cy7-conjugated CD3 (eBioscience, Vienna, Austria); allophycocyanin (APC)-conjugated CD38 (Biolegend, London, UK); APC-Fire 750-conjugated CD8α (Biolegend), CD20 (BD Biosciences, Erebodegem, Belgium); BV510-conjugated CD45 (BD Biosciences); Peridnin-chlorophyll Cy 5.5-conjugated CD4 (Biolegend); iFluor 555-conjugated MonoRab Rabbit Anti-Camelid VHH (Genscript by Bio-Connect, Huissen, The Netherlands); Vioblue-conjugated CD34 (MACS Milteyni Biotech).

Free full text: Click here

De Munter S., Van Parys A., Bral L., Ingels J., Goetgeluk G., Bonte S., Pille M., Billiet L., Weening K., Verhee A., Van der Heyden J., Taghon T., Leclercq G., Kerre T., Tavernier J, & Vandekerckhove B. (2020). Rapid and Effective Generation of Nanobody Based CARs using PCR and Gibson Assembly. International Journal of Molecular Sciences, 21(3), 883.

Publication 2020

PE-Cy7-conjugated CD3

Allophycocyanin Anti antibodies Chlorophyll Human Phycoerythrin Rabbit

Corresponding Organization : Ghent University

Other organizations : VIB-UGent Center for Medical Biotechnology

Top 5 similar protocols

Variable analysis

independent variables

Monoclonal antibodies used for surface marker staining: phycoerythrin (PE)-conjugated CD33, PE-Cy7-conjugated CD3, allophycocyanin (APC)-conjugated CD38, APC-Fire 750-conjugated CD8α and CD20, BV510-conjugated CD45, Peridnin-chlorophyll Cy 5.5-conjugated CD4, iFluor 555-conjugated MonoRab Rabbit Anti-Camelid VHH, Vioblue-conjugated CD34

dependent variables

Not explicitly mentioned

control variables

Staining of surface markers was performed as described earlier [42 (link)]

controls

No positive or negative controls were explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!