C2C12 cells were fixed and permeabilized as described [18 (link)], were blocked with PBS containing 1 % bovine serum albumin. Slides or cells were then immuno-stained with specific Rhodamine- or FITC-conjugated antibodies and nuclei were revealed with DAPI staining.
The MITO CytoPainter mitochondrial indicator is a hydrophobic compound that easily permeates intact live cells and becomes trapped in mitochondria after it enters the cells. This staining was performed on C2C12 live cells during the proliferative phase and all the differentiation phases.
Cell ROX® Oxidative Stress Reagents are fluorogenic probes designed to reliably measure ROS in live cells. The cell-permeable reagents are non-fluorescent or very faintly fluorescent while in a reduced state, and during oxidation exhibit a strong fluorogenic signal. Cell ROX® Orange Reagents are localized in the cytoplasm. This staining was performed on C2C12 live cells during the proliferative phase.
Slides were mounted with Moviol. Cells were observed using Nikon Eclipse 50I microscopy and images were captured using Nis-Elements D 4.00 software (Nikon Instruments Europe BV-Netherlands).
Data were displayed and analyzed using Adobe® Photoshop®CS4.
The MITO CytoPainter mitochondrial indicator is a hydrophobic compound that easily permeates intact live cells and becomes trapped in mitochondria after it enters the cells. This staining was performed on C2C12 live cells during the proliferative phase and all the differentiation phases.
Cell ROX® Oxidative Stress Reagents are fluorogenic probes designed to reliably measure ROS in live cells. The cell-permeable reagents are non-fluorescent or very faintly fluorescent while in a reduced state, and during oxidation exhibit a strong fluorogenic signal. Cell ROX® Orange Reagents are localized in the cytoplasm. This staining was performed on C2C12 live cells during the proliferative phase.
Slides were mounted with Moviol. Cells were observed using Nikon Eclipse 50I microscopy and images were captured using Nis-Elements D 4.00 software (Nikon Instruments Europe BV-Netherlands).
Data were displayed and analyzed using Adobe® Photoshop®CS4.
Free full text: Click here
