Quantitative Analysis of Newly Formed Bone

Calvaria explants from each group were stained using Stevenel’s Blue and counterstained with Van Gieson to visualize newly formed bone (NFB) tissue. Colored microscopic images of the medial (central) sagittal histologic sections were acquired under 10x magnification using Nikon Eclipse Ti-U camera with automatic stage, then merged using Nikon software to stich each image as one figure. The newly formed bone area (B.Ar.) and the total defect area (T.Ar.) were quantified using Image J software to calculate the NFB in % followed by the standardized protocol^{30 (link)}.

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Lee D.J., Lee Y.T., Zou R., Daniel R, & Ko C.C. (2017). Polydopamine-Laced Biomimetic Material Stimulation of Bone Marrow Derived Mesenchymal Stem Cells to Promote Osteogenic Effects. Scientific Reports, 7, 12984.

Publication 2017

Eclipse Ti-U camera

Calvaria Formed bone Microscopic Tissue

Corresponding Organization :

Other organizations : University of North Carolina at Chapel Hill

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Newly formed bone area (B.Ar.)
Total defect area (T.Ar.)

control variables

Histologic sections were acquired under 10x magnification using Nikon Eclipse Ti-U camera with automatic stage
Histologic sections were stained using Stevenel's Blue and counterstained with Van Gieson

controls

No positive or negative controls were explicitly mentioned in the provided information.

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