Corneal Transepithelial Electrical Resistance Measurement

Corneal TER was measured as previously described [16] (link). Briefly, a 1.0-mm-diameter Ag/AgCl electrode (Physiotech, Tokyo, Japan) was inserted into the anterior chamber through a small incision in the peripheral cornea, which had been made with an 18-gauge sharp needle (Terumo, Tokyo, Japan). Using biomedical adhesive (Alon-Alpha A; Sankyo), a 6.0-mm-internal diameter (0.28-cm² inner area) nitrile rubber O-ring (Union Packing; SAN-EI, Osaka, Japan) was fixed on the cornea. Then, 60 μL of HBSS was placed inside the ring at the center of the cornea, and the other electrode was carefully placed on the cornea. The TER was measured using a volt-ohm meter (EVOMX, World precision Instruments, Saeasota, FL), which generates ±20-μA alternating current (AC) square wave current at 12.5 Hz, and data were recorded using a thermal arraycorder (WR 300-8; Graphtech, Tokyo, Japan).

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Chen W., Dong N., Huang C., Zhang Z., Hu J., Xie H., Pan J, & Liu Z. (2014). Corneal Alterations Induced by Topical Application of Commercial Latanoprost, Travoprost and Bimatoprost in Rabbit. PLoS ONE, 9(3), e89205.

Publication 2014

18-gauge sharp needle EVOMX

Anterior chamber Cornea Hbss Needle Nitrile Rubber

Corresponding Organization :

Other organizations : Xiamen University

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Variable analysis

independent variables

Insertion of a 1.0-mm-diameter Ag/AgCl electrode into the anterior chamber through a small incision in the peripheral cornea
Fixing a 6.0-mm-internal diameter (0.28-cm2 inner area) nitrile rubber O-ring on the cornea using biomedical adhesive
Placing 60 μL of HBSS inside the ring at the center of the cornea
Carefully placing the other electrode on the cornea

dependent variables

Corneal transepithelial electrical resistance (TER)

control variables

Cornea
Experimental setup (e.g., incision, O-ring, HBSS volume, electrode placement)
Measurement technique (e.g., volt-ohm meter, AC square wave current at 12.5 Hz)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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