CD133+cells transfected with XRCC1 shRNA or negative control shRNA were cultured in 96 well plates (1 × 104 cells/well) overnight. On the second day, the cells were treated with 5-Fluorouracil (5-FU, final concentration of 0.1 mg/L) (APExBIO, United States) (Paschall et al., 2016 (link)). After 72 h, CCK-8 solution was added to each well, and the cultures were incubated at 37°C for 4 h. Then, the cultures were detected by use of a microplate reader.
CD133+cells transfected with XRCC1 shRNA or negative control shRNA were cultured in 6 well plates (2 × 105 cells/well) overnight. On the second day, the cells were treated with 5-Fluorouracil (5-FU, final concentration of 0.1 mg/L). After 72 h, cells were harvested to assess cell apoptosis by flow cytometry as above.
CD133+cells transfected with XRCC1 shRNA or negative control shRNA were cultured in 6 well plates (2 × 105 cells/well) overnight. On the second day, the cells were treated with 5-Fluorouracil (5-FU, final concentration of 0.1 mg/L). After 72 h, cells were harvested to assess cell apoptosis by flow cytometry as above.
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