Quantifying Neutrophil Extracellular Traps

NET formation was quantified by measuring histone area^{14 (link)}. Neutrophils were seeded onto coverslips and processed as described above for determination of NET structure. Images were collected using a Leica confocal microscope and processed using Leica Application Suite X and MetaMorph software. Briefly, NETs were visualized in at least five random fields (×40 magnification), signal intensity of histone per field was individually measured and the pixels count for each image was converted into area (μm²) using a calibration unit (0.8333). Mean histone area (μm²) was determined from the five independent fields.

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Sung P.S., Huang T.F, & Hsieh S.L. (2019). Extracellular vesicles from CLEC2-activated platelets enhance dengue virus-induced lethality via CLEC5A/TLR2. Nature Communications, 10, 2402.

Publication 2019

confocal microscope Application Suite X MetaMorph software

Confocal microscope Histone Net formation Nets Neutrophils

Corresponding Organization :

Other organizations : National Yang Ming University Hospital, National Yang Ming Chiao Tung University, Mackay Medical College

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Variable analysis

independent variables

NET formation

dependent variables

Histone area

control variables

Neutrophils seeded onto coverslips
Processing as described above for determination of NET structure

controls

No explicit controls mentioned

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