HDAC3 Modulation Regulates AQP4 and miR-130a

HDAC3 siRNA duplex (Guangzhou RiboBio Co., Ltd.) or RGFP966 (Selleck Chemicals Co., Ltd.) was used to interfere with endogenous HDAC3 mRNA levels. siRNA was performed with siRNA transfection reagent (Guangzhou RiboBio Co., Ltd.) as we have described in detail previously (Zhang et al., 2017 (link)). Cells were incubated in 6-well culture microplates at 37°C with antibiotic-free medium containing 10 μM RGFP966. After 24 h, the expression of AQP4 mRNA and protein, and miR-130a levels were investigated by the above methods. Untreated cells and non-specific siRNA (scrambled siRNA; Guangzhou RiboBio Co., Ltd.) were used as controls. This experiment was repeated three times and performed in duplicate.

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Zhang J., Zhan Z., Li X., Xing A., Jiang C., Chen Y., Shi W, & An L. (2017). Intermittent Fasting Protects against Alzheimer’s Disease Possible through Restoring Aquaporin-4 Polarity. Frontiers in Molecular Neuroscience, 10, 395.

Publication 2017

HDAC3 siRNA duplex RGFP966 siRNA transfection reagent scrambled siRNA

Antibiotic Cells Mrna Protein Rgfp966 Sirna Transfection

Corresponding Organization : China Medical University

Other organizations : Jinzhou Medical University

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Variable analysis

independent variables

HDAC3 siRNA duplex (Guangzhou RiboBio Co., Ltd.)
RGFP966 (Selleck Chemicals Co., Ltd.)

dependent variables

AQP4 mRNA expression
AQP4 protein expression
MiR-130a levels

control variables

Antibiotic-free medium
10 μM RGFP966 concentration
Incubation time of 24 h
Incubation temperature of 37°C
Culture in 6-well microplates

controls

Untreated cells (negative control)
Non-specific siRNA (scrambled siRNA; Guangzhou RiboBio Co., Ltd.) (negative control)

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