Quantification of Recombinant Receptor Expression

Flow cytometry after labeling cells with Phycoerythrin-conjugated anti-FLAG antibody (Biolegend, 637310), Alexa 647-conjugated anti-FLAG antibody (R&D, IC8529R), Alexa 488-conjugated anti-Myc antibody (R&D, IC3696G) or FITC-conjugated anti-HA antibody (Sigma-Aldrich, H7411) was used to quantify recombinant receptor expression levels in HEK293T cells, as described^{15 (link),30 (link),31 (link)}. At least 10,000 cells/sample were recorded and analyzed with the FlowJo software (Tree Star).

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Gao X., Albee L.J., Volkman B.F., Gaponenko V, & Majetschak M. (2018). Asymmetrical ligand-induced cross-regulation of chemokine (C-X-C motif) receptor 4 by α1-adrenergic receptors at the heteromeric receptor complex. Scientific Reports, 8, 2730.

Publication 2018

FITC-conjugated anti-HA antibody

Anti antibody Cells Fitc Flow cytometry Phycoerythrin Tree

Corresponding Organization : Loyola University Chicago

Other organizations : Medical College of Wisconsin, University of Illinois at Chicago

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Variable analysis

independent variables

Phycoerythrin-conjugated anti-FLAG antibody (Biolegend, 637310)
Alexa 647-conjugated anti-FLAG antibody (R&D, IC8529R)
Alexa 488-conjugated anti-Myc antibody (R&D, IC3696G)
FITC-conjugated anti-HA antibody (Sigma-Aldrich, H7411)

dependent variables

Recombinant receptor expression levels in HEK293T cells

control variables

At least 10,000 cells/sample were recorded and analyzed with the FlowJo software (Tree Star)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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