Quantification of Recombinant Receptor Expression
Corresponding Organization : Loyola University Chicago
Other organizations : Medical College of Wisconsin, University of Illinois at Chicago
Variable analysis
- Phycoerythrin-conjugated anti-FLAG antibody (Biolegend, 637310)
- Alexa 647-conjugated anti-FLAG antibody (R&D, IC8529R)
- Alexa 488-conjugated anti-Myc antibody (R&D, IC3696G)
- FITC-conjugated anti-HA antibody (Sigma-Aldrich, H7411)
- Recombinant receptor expression levels in HEK293T cells
- At least 10,000 cells/sample were recorded and analyzed with the FlowJo software (Tree Star)
- No positive or negative controls were explicitly mentioned in the provided information.
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!