Flow cytometry after labeling cells with Phycoerythrin-conjugated anti-FLAG antibody (Biolegend, 637310), Alexa 647-conjugated anti-FLAG antibody (R&D, IC8529R), Alexa 488-conjugated anti-Myc antibody (R&D, IC3696G) or FITC-conjugated anti-HA antibody (Sigma-Aldrich, H7411) was used to quantify recombinant receptor expression levels in HEK293T cells, as described15 (link),30 (link),31 (link). At least 10,000 cells/sample were recorded and analyzed with the FlowJo software (Tree Star).
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