Total RNA was extracted using Trizol reagent. Reverse transcription and SYBR green qPCR was performed using a kit from Vazyme lnc, Nanjing China. and carried out by the Roche LightCycler 480 RT-PCR System. β-actin was placed as a loading control, and the delta delta Ct (2−ΔΔCt) method was used to calculate the fold changes. Primers were used from the literature, as shown in the following [60 (link)]: BCL-w F: AGT TCG AGA CCC GCT TCC R: CCC GTC CCC GTA TAG AGC BCL-xl F: CTG AAT CGG AGA TGG AGA CC R: TGG GAT GTC AGG TCA CTG AA ACTB F: GCC CTG AGG CAC TCT TCC A R: CCA GGG CAG TGA TCT CCT TCT.
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